Journal: iScience

Article Title: Circadian disruption during fetal development promotes pathological cardiac remodeling in male mice

doi: 10.1016/j.isci.2024.109008

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Article Snippet: Mouse Brain Natriuretic Peptide (BNP) ELISA test Kit , Cloud-Clone , Cat# SEA541Mu.

Techniques: Recombinant, Virus, Fluorescence, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Molecular Pain

Article Title: Inefficient constitutive inhibition of P2X3 receptors by brain natriuretic peptide system contributes to sensitization of trigeminal sensory neurons in a genetic mouse model of familial hemiplegic migraine

doi: 10.1177/1744806916646110

Figure Lengend Snippet: BNP and NPR-A in WT and KI TGs. (a) Representative immunocytochemical examples of endogenous NPR-A expression in WT (left panel) and KI (right panel) mouse TG cultures. Nuclei are visualized with DAPI (blue); scale bar = 50 µm. Note extensive costaining of NPR-A (red) and β-tubulin (green). (b) The histograms quantify percentage of NPR-A positive cells in WT and KI cultures ( n = 4; * p < 0.05, Kruskal–Wallis test). (c) Western blot showing protein expression of NPR-A in TG from P30 mice (right) and TG cultures from P12 mice (left). β-Actin was used as a loading control. Histograms (bottom) quantify NPR-A relative optical density values for each examined group. (d) ELISA-based quantification of intracellular cGMP in TG cultures from WT and KI mice. Cultures were treated with BNP (500 ng/ml for 5, 30, or 60 min) and/or anantin (500 nM, 30 min) or vehicle (indicated as 0). Note similar dynamics of cGMP increase after BNP application and similar decrease after treatment with anantin alone (500 nM) throughout. Data were normalized to the total protein content in each sample and are presented as mean value ± standard deviation ( n = 3 in which samples were run in duplicate; * p < 0.05, Kruskal–Wallis test). (e) Immunocytochemical analysis of BNP (red) in WT and KI mouse TG culture. Nuclei are visualized with DAPI (blue). Scale bar, 15 µm. (f) Histograms show percent value of BNP-positive cells in WT and KI populations (DAPI-labeled cells).

Article Snippet: To determine BNP concentration in medium samples, a commercial ELISA kit for mouse BNP was used (Abnova, Hidelberg, Germany) following the instructions of the manufacturer, as previously reported.

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation, Labeling